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KMID : 0381120220440111405
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Genes and Genomics
2022 Volume.44 No. 11 p.1405 ~ p.1414
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Circular RNA expression profile of systemic lupus erythematosus and its clinical significance as a potential novel biomarker
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Li Wenyu
Fan Runge
Zhou Cheng
Wei Yue
Lin Shunsheng
Wen Sijian
Zeng Wen
Hou Wei
Zhao Cheng
Lin Youkun
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Abstract
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Background: Circular RNAs (circRNAs) are a class of endogenous noncoding RNAs that are more abundant, specific, and highly organized than linear RNAs. Increasing evidence supports that circRNAs may serve as diagnostic biomarkers in many diseases, but their potential as biomarkers in systemic lupus erythematosus (SLE) remains unclear.
Objective: We investigated the critical circRNAs involved in SLE progression and explored their potential application as biomarkers in SLE.
Method: RNA sequencing was conducted on peripheral blood mononuclear cells (PBMCs) from 4 SLE patients and 4 healthy volunteers. CircRNA profile data were analyzed to identify differentially expressed circRNAs and visualized via R software. After screening, qPCR analysis of target circRNA expression was performed using PBMCs from 31 SLE patients and 35 healthy volunteers. Correlations between circRNA expression levels and the SLEDAI score were assessed via Spearman correlation analysis. Finally, the performance of circRNAs as biomarkers in SLE was examined by receiver operating characteristic curve analysis.
Results: The result identified six differentially expressed circRNAs between SLE patients and healthy controls: hsa_circ_0006689, hsa_circ_0070562, hsa_circ_0006117, hsa_circ_0007683, hsa_circ_0042519, and hsa_circ_0008647. The validation analysis showed differing relative expression levels of hsa_circ_0007683, hsa_circ_0042519, hsa_circ_0008647, and hsa_circ_0006689 between SLE patients and healthy volunteers (P?
Conclusion: Our results suggest hsa_circ_0006689 may be a useful circRNA biomarker for SLE diagnosis and prognosis.
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KEYWORD
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Systemic lupus erythematosus (SLE), circRNAs, RNA sequencing, hsa_circ_0006689
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